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sigmoidal dose response curve-fitting program graphpad prizm  (GraphPad Software Inc)
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Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.
Prizm 2.01, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nonlinear curve-fitting program for sigmoidal dose-responses  (GraphPad Software Inc)
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Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.
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sigmodial dose response curve-fitting models  (GraphPad Software Inc)
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Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.
Sigmodial Dose Response Curve Fitting Models, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.
Prizm, supplied by Claritas Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.
Prizm 5, supplied by TIBCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.
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software graphpad prizm  (GraphPad Software Inc)
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Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.
Software Graphpad Prizm, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.

Journal:

Article Title: ?? protomers of Na + ,K + -ATPase from microsomes of duck salt gland are mostly monomeric: Formation of higher oligomers does not modify molecular activity

doi:

Figure Lengend Snippet: Effects of varied concentrations of ouabain and ouabain derivatives on PNPPase activity. Purified Na+,K+-ATPase was incubated at 20 μg/ml with varied concentrations of ouabain, AO, FO, and NBDO for 30 min at room temperature, and the PNPPase activity was measured as described in Methods. The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego). The fitted parameters and symbols are: ○ = ouabain, EC50 = 6.4 nM; ● = AO, EC50 = 64 nM; □ = NBDO, EC50 = 210 nM; ■ = FO, EC50 = 860 nM.

Article Snippet: The data were fitted to a sigmoidal dose-response curve by using prizm 2.01 (GraphPad, San Diego).

Techniques: Activity Assay, Purification, Incubation